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Dr. Nilesh M. Mahajan

Dadasaheb Balpande College of Pharmacy, India

Title: Development and evaluation of wound healing hydrogel from chicken feather protein

Biography

Biography: Dr. Nilesh M. Mahajan

Abstract

Chicken feathers are the largest bio-waste produced across the world. Available uncontrolled disposal methods are not ecofriendly. Chicken feathers consists of about 91% Keratin protein. Keratin is a cysteine-rich structural proteins with unique characteristics of bioactivity, biocompatibility, biodegradability, and natural abundance. Protein component contain a defined three dimensional microstructure that aids in cellular proliferation and cell guided tissue formation. Genistein is a secondary metabolite isoflavones found in soybean. Genistein protects human dermal fibroblasts from oxidative stress-induced collagen biosynthesis inhibition thus acting as a epidermal growth factor. In this research Keratin was extracted, purified and charcterized to used in wound helaing hydrogel containig Genistein. Purified Keratin was tested for confirmational analysis by FTIR, SEM, SDS-PAGE, Amino acid profiling. Extracted Keratin was found to retain all the characterisitic values with reference standard. The formulated product was then tested for efficacy by in-vivo wound healing activity and saftey. The optimized wound healing formulation of Keratin - Genistein gel showed effective results of gel strength, pH, viscosity, spreadibility, drug contents. HPTLC study shows presence of both components in the gel without any interactions. Stability study indicates that the formulation was fairly stable. The results of in-vivo study indicated that feather keratin hydrogel significantly accelerated the wound healing compared to untreated group. Organ toxicity study revels safety of developed combination product.